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pet28b rfxcas13d his  (Addgene inc)


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    Structured Review

    Addgene inc pet28b rfxcas13d his
    Pet28b Rfxcas13d His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28b+rfxcas13d+his/pET-28b-RfxCas13d-His+(Plasmid+%23141322)/bio_rxiv__64898__2026__03__13__711615-220-10-11
    Average 93 stars, based on 21 article reviews
    pet28b rfxcas13d his - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Massively parallel profiling of RNA-targeting CRISPR-Cas13d
    Article Snippet: .. Ruminococcus flavefaciens Cas13d (RfxCas13d, or CasRx) was subcloned into a pET19-based plasmid with an N-terminal 6xHis-TwinStrep-SUMO fusion to generate the plasmid pIF1034 from pET28b-RfxCas13d-His (Addgene #141322). ..

    Article Title: Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos
    Article Snippet: pT3TS-RfxCas13d-NLS , , Addgene plasmid #141321. .. pET28B-RfxCas13d-His , , Addgene plasmid #141322. ..

    Article Title: Massively parallel profiling of RNA-targeting CRISPR-Cas13d.
    Article Snippet: .. Ruminococcus flavefaciens Cas13d (RfxCas13d, or CasRx) was subcloned into a pET19-based plasmid with an N-terminal 6xHis-TwinStrep-SUMO fusion to generate the plasmid pIF1034 from pET28b-RfxCas13d-His (Addgene #141322). ..

    Transformation Assay:

    Article Title: Laminin and Fibronectin Cooperate to Guide Endothelial Self-Organization During Intersegmental Vessel Formation
    Article Snippet: .. Briefly, E. coli Rosetta(DE3) pLysS (Novagen) cells were transformed with pET28b-RfxCas13d-His (Addgene 141322), induced with 0.1 mM IPTG, and lysed by sonication. ..

    Sonication:

    Article Title: Laminin and Fibronectin Cooperate to Guide Endothelial Self-Organization During Intersegmental Vessel Formation
    Article Snippet: .. Briefly, E. coli Rosetta(DE3) pLysS (Novagen) cells were transformed with pET28b-RfxCas13d-His (Addgene 141322), induced with 0.1 mM IPTG, and lysed by sonication. ..



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